I disagree with the prior comments. The Accuri C6 cytometer in my lab has some problems, and we need some help. 1) Open instrument 2) Remove hex bolts holding instrument guts in chasis. Figure 5-4. and in case, if i need to stain with antibody, than i could stain with it for 30 mints only or i need to stain it with antibody till i used it ???? Accuri Cytometers ii 7820041-01 Rev-A 4.12.2 Correcting Fluorescence Spillover ..... 55 1.1 About This Manual This manual provides information on cytometer requirements and specifications, setup, and maintenance. Has anyone demoed or purchased this machine? Any thoughts on the FACsCelesta flow cytometer compared with the FACsCanto II? BD Accuri C6 / plus -- Problem again and again? This protocol also assumes a completed assay in a 96-well plate, with particles suspended in Run buffer. Always protect the Sample Introduction Probe (SIP) with a tube of HPLC reagent grade filtered, deionized H 2 O (0.22 µm filter), or equivalent, when the cytometer is not running samples. 3) Remove back plate. 98 0 obj <>/Filter/FlateDecode/ID[<00FCDA4AADBD4BAE9B9E563DAC7F2050><401A5750BC3C5B448D6FC8BE2B4CA82B>]/Index[73 40]/Info 72 0 R/Length 112/Prev 808970/Root 74 0 R/Size 113/Type/XRef/W[1 2 1]>>stream Each with lock and regular washer a. The BD Accuri C6 Plus offers plug-and-play simplicity and walk-away capabilities thanks to turnkey, automated software. 26. The BD Accuri C6 has a 1-minute UNCLOG cycle which may be run any time an obstruction is suspected. also are there other programs out there? Place an empty tube on the sample port and select the UNCLOG button in the workspace. I was wondering if we were the only ones with this type of problem. Manuals and User Guides for BD Accuri C6. INFO BELOW ... SB3820-C6 Model Information - Vizio As always, we want to … Unplug. Remove and discard the black SIP cover and place a tube containing at least 2mL of filtered, de-ionized water on the SIP. To me BD FACSVerse is better than BD acuuri. The BD Accuri C6 Plus only weighs only 30 pounds and has a compact footprint of 11 x 14.75 x 16.5 inches, but empowers a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. It also includes common troubleshooting help and service information. 27. So the choice should be addressed by the kind of flow experiments you plan to do. Gently homogenize the blood sample inside heparin blood collection tube. Use a Wrist Grounding Strap. For walkaway convenience, the optional BD CSampler™ accessory offers reliable and easy-to-use automation. The BD Accuri C6 flow cytometer is easy to use and allows student access to the science of flow cytometry. I have used FACScaliber. 11. It will be helpful if the user interface is similar to the Cell quest. I study few protocols, somewhere they said i need to fix it with PBS and when i used than i will need to stain with antibody. What is the best? To install BD Accuri C6 Software, run BDAccuriC6SoftwareInstaller.exe. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. I want to identify neutrophil in rat lung tissue by immunohistochemistry. however, somewhere i read that i need to wash with PBS, stain with antibody for 30 mints and than wash with PBS to store. What are your thoughts? We have had our Accuri since 2008 and it has been great. we have figured it out of how to solve the problems with BD Accuri C6 flow cytometer. Thank you very much. The Accuri C6 Flow Cytometer does not produce aerosols during normal operation. The FACSVerse is a far superior instrument to the C6. I recommend you BD FACSVerse. The unusually high dynamic range of the ADC allows simultaneous analysis of species for which C-values span almost the entire range of the described angiosperms. The software is complicated but Diva (the software of Canto II) was any better...You can also determine the concentration of your sample without calibration beads or calibrate fluorescece/compensate it is not necessary anymore (it is automatically done by cheking Quality Control with dedicated beads, that you have to use once a day). Can anyone recommend a good free FACS analysing software? Still I would love to know the better one. What is the best marker for neutrophil identification by immunohistochemistry? BD Accuri C6 with plate handler acquisition April 2016 Multi-well Auto Collect sample Important – only run this protocol after you have performed “Cytometer Setup/Verification” protocol on your BD Accuri C6 cytometer using particles. This manual provides information on cytometer requirements and specifications, setup, and maintenance. The downside to the FACSVerse is that it had some issues when it first came to market, but I believe many of those issues have been ironed-out. From basic to more challenging applications, the BD Accuri™ C6 Plus personal flow cytometer is a powerful benchtop tool that provides you with the versatility and the analytical power of a cytometer twice its size. Software options … The BD Accuri C6 Software installer is on the CD that came with the C6. BD Accuri C6 Plus software features an intuitive user interface. We sent the Accuri C6 Plus (received new Nov 2016 and this issue happened July 2018) into BD to be properly diagnosed and find out the cost for repair. For information on using BD Accuri C6 Software, see the BD Accuri C6 Software User Guide. Sort by: Instrument Options (1 of 1) results found. To be honest with you although the Accuri C6 has still some minor issues that could be sorted out (and I´m sure they´re working on it), even with those problems, I love it, as I said before it´s great for small groups and labs, but also a great complement for a core facility. Are there anyone has different protocol working good? Status:RUO Cat No.:660519. What is the best marker for identification of neutrophil in lung tissue by immunohistochemistry? For information on installing and using the BD CSampler™, see the BD Accuri BD Accuri™ C6 Flow Cytometer Instrument Manual system, etc.) Hold the module with one hand and screw in the front right mounting bolt to secure the BD CSampler to the BD Accuri C6, tightening with the tool provided (Figure 6). 6. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. Our machine is still sitting in San Jose at BD over a year later because we haven't decided if it's worth paying for the repairs. It depends on what you want to do. Our machine is still sitting in San Jose at BD over a year later because we haven't decided if it's worth paying for the repairs. The Accuri is an excellent machine. Fluid should accumulate in the tube. If you’re new to flow cytometry, and/or use one of the modern benchtop flow cytometers which don’t require, or even allow, you to mess with the guts of the instrument, you may not care much about how flow cytometers work. One of the main advantages of this is the ability to use a variety of sample tubes/plates from which to collect samples. %PDF-1.6 %���� I am using cancer cell lines for my study. Direct counts on the BD Accuri C6 correlate highly with counting beads, and are more precise than hemocytometer counts. FCS 2 (Verse) is not compatible with free software like Flowing 2 etc. VERSATILE. 3 on bottom. Align the front of the BD CSampler so that the holes on the module are lined up with the three threaded holes in the BD Accuri C6. I also have experience with the Accuri. You may also check the Attune Acoustics Focusing Cytometer (, We have a 3 laser Verse with automatic sample. Another very important issue is the amount of events you think you have to acquire for each tube. 112 0 obj <>stream CONVENIENCE IN YOUR LAB. I'm a new comer of the immunology field. Browse for Folder Dialog Box. If you want to know more about the Accuri you can contact me. The BD Accuri C6 Plus personal flow cytometer is the newest generation of the BD Accuri platform. As with anything new there were a few bugs and the software takes a bit of getting used to. Preventative Maintenance on the BD Accuri™ C6 Plus - YouTube Status:RUO Cat No.:661391. A month later, we have (again) a problem with the valves. Figure 5-1. BD Accuri C6 cytometer simplifies system maintenance with automatic cleaning cycles on instrument shutdown. 73 0 obj <> endobj I'm completely agree with the words of Ryan Duggan. C6 User Guide BD Accuri™ C6 Flow Cytometer Instrument Manual NOKIA C6-00 USER MANUAL Pdf Download. 27. 1. Introduction The BD Accuri® C6 personal flow cytometer can simplify cell analysis by counting absolute cell numbers directly (per unit sample volume). 26. Quick View. see your device’s user manual. The FACSVerse was designed to showcase the next generation of BD engineering, and although that comes with a few growing pains, it really does allow for some very nice advantages. I am analysing my data with cell quest (which is not my favorite program) I used to use Flowjo (which rocks in my opinion) but have no means to ever get this. Just few consideration more. Our lab is looking to purchase a flow cytometer. The BD Accuri™ C6 flow cytometer is ideally suited for determining C-values for new plant species. By looking excitation spectra I would say no (~5% excitation), but its maybe enough in practice ? (pic 2), Hope you guys can help me with this. Verse is able to acquire and process millions of events, C6 do not. It also includes common troubleshooting … Even new flow cytometry users find it so easy to learn and use that most can collect and analyze data in less than an hour. Planning to have a flowcytometer. It uses a bunch of new technology that BD has built to address usability (software) and reliability (hardware). The Accuri C6 cytometer in my lab has some problems, and we need some help. I have been doing flow for over 25 years and used many machines and actually found the Accuri to be far superior for working with large cells such as macrophages. BD Accuri™ C6 Plus Workstation Computer and Software. Mendeley or Endnote as a reference manager software? Originally we were going to purchase the FACsCanto II however the reps mentioned the recently available FACsCelesta. Update Driver Software - Cytometer, Second Dialog Box. BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express™ software and FlowJo™ software. I was preparing 6x laemli buffer with mercaptoethanol as giwen below: Before use add 1/8th volume of β-mercaptoethanol. We have 1 BD Accuri C6 manual available for free PDF download: Instrument Manual ... Troubleshooting and Technical Support. A BD Biosciences technical bulletin contains recommendations, tips, techniques, and troubleshooting suggestions to help maximize the accuracy of cell counts and concentrations on the BD Accuri C6. The two instruments are not really comparable. Such counts are essential in many research and clinical applications, including enumerating leucocytes, B cells, T cells, and platelets in human blood, measuring of cultured cell lines. endstream endobj 74 0 obj <>>> endobj 75 0 obj <> endobj 76 0 obj <>/Font<>/ProcSet[/PDF/Text]/XObject<>>>/Rotate 0/TrimBox[0.0 0.0 612.0 792.0]/Type/Page>> endobj 77 0 obj <>stream On the other hand, if you need flow cytometer only for simple and repetitive experiments C6 might be enough. The BD Accuri C6 Plus personal flow cytometer. N {����>�'p )L �` According to protocols, I will collect the cells and wash in PBS, than i will stain with a antibody (Fab fragment antibody) for flow cytometry. I want to store my cells, and want to run FACS after few days. I played around with WinMDI but something weird happend when I comparing the data analysed in cell quest. Stain and fix cell for flow analysis, and easy to train students on until! 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